r studio desktop ide 1.0.143 (RStudio)
90
Structured Review
RStudio
r studio desktop ide 1.0.143
R Studio Desktop Ide 1.0.143, supplied by RStudio, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/desktop+ide+1%2E0%2E143/desktop+ide+1+0+143/pmc07108696-55-2-0
Average 90 stars, based on 1 article reviews
R Studio Desktop Ide 1.0.143, supplied by RStudio, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/desktop+ide+1%2E0%2E143/desktop+ide+1+0+143/pmc07108696-55-2-0
Average 90 stars, based on 1 article reviews
r studio desktop ide 1.0.143 - by Bioz Stars,
2026-10
90/100 stars
Images
1) Product Images from "Detection of Marker-Free Precision Genome Editing and Genetic Variation through the Capture of Genomic Signatures"
Article Title: Detection of Marker-Free Precision Genome Editing and Genetic Variation through the Capture of Genomic Signatures
Journal: Cell reports
doi: 10.1016/j.celrep.2020.02.068
Figure Legend Snippet: KEY RESOURCES TABLE
Techniques Used: Virus, Subcloning, Recombinant, Staining, Magnetic Beads, DNA Extraction, PCR Cloning, Sequencing, Cloning, Plasmid Preparation, Expressing, Software
Related Articles
Subcloning:Article Title: CRISPR-Mediated Base Editing Enables Efficient Disruption of Eukaryotic Genes through Induction of STOP Codons Article Snippet: Article Title: CRISPR-Mediated Base Editing Enables Efficient Disruption of Eukaryotic Genes through Induction of STOP Codons Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Mouse monoclonal anti-SMARCAL1 (A-2) Santa Cruz Cat#sc-376377; RRID: AB_10987841 Mouse monoclonal anti-GAPDH Novus Cat#NB300-221; RRID: AB_10077627 Anti-Mouse IgG, HRP-linked GE Healthcare Cat#NA931-1ML; RRID: AB_772210 Bacterial and Virus Strains Subcloning Efficiency DH5α Life Technologies Cat#18265-017 Chemicals, Peptides, and Recombinant Proteins Transfection reagent: TransIT-293 Mirus Cat#MIR 2700 Carbenicillin Goldbio Cat#C-103-25 Ouabain octahydrate Sigma Cat#03125-1G Q5 High-Fidelity DNA polymerase NEB Cat#M0491L Critical Commercial Assays Quick Extract DNA Extraction solution Epicenter Cat#QE09050 Zero BLUNT II TOPO PCR Cloning kit Life Technologies Cat#450245 SuperSignal West Pico Chemiluminescent Substrate Thermo Fisher Scientific Cat#34087 Deposited Data Unprocessed images of gels and blots This paper; Mendeley Data http://dx.doi.org/10.17632/jw5rjmypy2.1 Annotated sgSTOPs for 8 organisms and 6 PAMs This paper; Mendeley Data http://www.ciccialab-database.com/istop ; http://dx.doi.org/10.17632/xbdtvf6bvj.1 Experimental Models: Cell Lines Human: HEK293T ATCC CRL-11268 Oligonucleotides Primers for PCR This paper Table S5 Oligonucleotides for sgRNA cloning This paper Table S5 Recombinant DNA Plasmid: eSpCas9(1.1) Addgene Addgene Plasmid #71814 Plasmid: MLM3636 Addgene Addgene Plasmid #43860 Plasmid: B52 (containing 2 empty sgRNAs-expressing cassettes) This paper Available in Addgene Plasmid: B270 (containing sgRNA targeting ATP1A1 + empty sgRNA-expressing cassette) This paper Available in Addgene Plasmid: pCMV-BE3 Addgene Addgene Plasmid #73021 Software and Algorithms iSTOP R package 0.1.0 This paper https://github.com/CicciaLab/iSTOP/tree/0.1.0 iSTOP reproducible analysis 0.1.1 This paper https://github.com/CicciaLab/iSTOP-paper/tree/0.1.1 R 3.4.1 The R project for statistical computing https://www.r-project.org RStudio Recombinant:Article Title: CRISPR-Mediated Base Editing Enables Efficient Disruption of Eukaryotic Genes through Induction of STOP Codons Article Snippet: Article Title: CRISPR-Mediated Base Editing Enables Efficient Disruption of Eukaryotic Genes through Induction of STOP Codons Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Mouse monoclonal anti-SMARCAL1 (A-2) Santa Cruz Cat#sc-376377; RRID: AB_10987841 Mouse monoclonal anti-GAPDH Novus Cat#NB300-221; RRID: AB_10077627 Anti-Mouse IgG, HRP-linked GE Healthcare Cat#NA931-1ML; RRID: AB_772210 Bacterial and Virus Strains Subcloning Efficiency DH5α Life Technologies Cat#18265-017 Chemicals, Peptides, and Recombinant Proteins Transfection reagent: TransIT-293 Mirus Cat#MIR 2700 Carbenicillin Goldbio Cat#C-103-25 Ouabain octahydrate Sigma Cat#03125-1G Q5 High-Fidelity DNA polymerase NEB Cat#M0491L Critical Commercial Assays Quick Extract DNA Extraction solution Epicenter Cat#QE09050 Zero BLUNT II TOPO PCR Cloning kit Life Technologies Cat#450245 SuperSignal West Pico Chemiluminescent Substrate Thermo Fisher Scientific Cat#34087 Deposited Data Unprocessed images of gels and blots This paper; Mendeley Data http://dx.doi.org/10.17632/jw5rjmypy2.1 Annotated sgSTOPs for 8 organisms and 6 PAMs This paper; Mendeley Data http://www.ciccialab-database.com/istop ; http://dx.doi.org/10.17632/xbdtvf6bvj.1 Experimental Models: Cell Lines Human: HEK293T ATCC CRL-11268 Oligonucleotides Primers for PCR This paper Table S5 Oligonucleotides for sgRNA cloning This paper Table S5 Recombinant DNA Plasmid: eSpCas9(1.1) Addgene Addgene Plasmid #71814 Plasmid: MLM3636 Addgene Addgene Plasmid #43860 Plasmid: B52 (containing 2 empty sgRNAs-expressing cassettes) This paper Available in Addgene Plasmid: B270 (containing sgRNA targeting ATP1A1 + empty sgRNA-expressing cassette) This paper Available in Addgene Plasmid: pCMV-BE3 Addgene Addgene Plasmid #73021 Software and Algorithms iSTOP R package 0.1.0 This paper https://github.com/CicciaLab/iSTOP/tree/0.1.0 iSTOP reproducible analysis 0.1.1 This paper https://github.com/CicciaLab/iSTOP-paper/tree/0.1.1 R 3.4.1 The R project for statistical computing https://www.r-project.org RStudio Transfection:Article Title: CRISPR-Mediated Base Editing Enables Efficient Disruption of Eukaryotic Genes through Induction of STOP Codons Article Snippet: Article Title: CRISPR-Mediated Base Editing Enables Efficient Disruption of Eukaryotic Genes through Induction of STOP Codons Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Mouse monoclonal anti-SMARCAL1 (A-2) Santa Cruz Cat#sc-376377; RRID: AB_10987841 Mouse monoclonal anti-GAPDH Novus Cat#NB300-221; RRID: AB_10077627 Anti-Mouse IgG, HRP-linked GE Healthcare Cat#NA931-1ML; RRID: AB_772210 Bacterial and Virus Strains Subcloning Efficiency DH5α Life Technologies Cat#18265-017 Chemicals, Peptides, and Recombinant Proteins Transfection reagent: TransIT-293 Mirus Cat#MIR 2700 Carbenicillin Goldbio Cat#C-103-25 Ouabain octahydrate Sigma Cat#03125-1G Q5 High-Fidelity DNA polymerase NEB Cat#M0491L Critical Commercial Assays Quick Extract DNA Extraction solution Epicenter Cat#QE09050 Zero BLUNT II TOPO PCR Cloning kit Life Technologies Cat#450245 SuperSignal West Pico Chemiluminescent Substrate Thermo Fisher Scientific Cat#34087 Deposited Data Unprocessed images of gels and blots This paper; Mendeley Data http://dx.doi.org/10.17632/jw5rjmypy2.1 Annotated sgSTOPs for 8 organisms and 6 PAMs This paper; Mendeley Data http://www.ciccialab-database.com/istop ; http://dx.doi.org/10.17632/xbdtvf6bvj.1 Experimental Models: Cell Lines Human: HEK293T ATCC CRL-11268 Oligonucleotides Primers for PCR This paper Table S5 Oligonucleotides for sgRNA cloning This paper Table S5 Recombinant DNA Plasmid: eSpCas9(1.1) Addgene Addgene Plasmid #71814 Plasmid: MLM3636 Addgene Addgene Plasmid #43860 Plasmid: B52 (containing 2 empty sgRNAs-expressing cassettes) This paper Available in Addgene Plasmid: B270 (containing sgRNA targeting ATP1A1 + empty sgRNA-expressing cassette) This paper Available in Addgene Plasmid: pCMV-BE3 Addgene Addgene Plasmid #73021 Software and Algorithms iSTOP R package 0.1.0 This paper https://github.com/CicciaLab/iSTOP/tree/0.1.0 iSTOP reproducible analysis 0.1.1 This paper https://github.com/CicciaLab/iSTOP-paper/tree/0.1.1 R 3.4.1 The R project for statistical computing https://www.r-project.org RStudio DNA Extraction:Article Title: CRISPR-Mediated Base Editing Enables Efficient Disruption of Eukaryotic Genes through Induction of STOP Codons Article Snippet: Article Title: CRISPR-Mediated Base Editing Enables Efficient Disruption of Eukaryotic Genes through Induction of STOP Codons Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Mouse monoclonal anti-SMARCAL1 (A-2) Santa Cruz Cat#sc-376377; RRID: AB_10987841 Mouse monoclonal anti-GAPDH Novus Cat#NB300-221; RRID: AB_10077627 Anti-Mouse IgG, HRP-linked GE Healthcare Cat#NA931-1ML; RRID: AB_772210 Bacterial and Virus Strains Subcloning Efficiency DH5α Life Technologies Cat#18265-017 Chemicals, Peptides, and Recombinant Proteins Transfection reagent: TransIT-293 Mirus Cat#MIR 2700 Carbenicillin Goldbio Cat#C-103-25 Ouabain octahydrate Sigma Cat#03125-1G Q5 High-Fidelity DNA polymerase NEB Cat#M0491L Critical Commercial Assays Quick Extract DNA Extraction solution Epicenter Cat#QE09050 Zero BLUNT II TOPO PCR Cloning kit Life Technologies Cat#450245 SuperSignal West Pico Chemiluminescent Substrate Thermo Fisher Scientific Cat#34087 Deposited Data Unprocessed images of gels and blots This paper; Mendeley Data http://dx.doi.org/10.17632/jw5rjmypy2.1 Annotated sgSTOPs for 8 organisms and 6 PAMs This paper; Mendeley Data http://www.ciccialab-database.com/istop ; http://dx.doi.org/10.17632/xbdtvf6bvj.1 Experimental Models: Cell Lines Human: HEK293T ATCC CRL-11268 Oligonucleotides Primers for PCR This paper Table S5 Oligonucleotides for sgRNA cloning This paper Table S5 Recombinant DNA Plasmid: eSpCas9(1.1) Addgene Addgene Plasmid #71814 Plasmid: MLM3636 Addgene Addgene Plasmid #43860 Plasmid: B52 (containing 2 empty sgRNAs-expressing cassettes) This paper Available in Addgene Plasmid: B270 (containing sgRNA targeting ATP1A1 + empty sgRNA-expressing cassette) This paper Available in Addgene Plasmid: pCMV-BE3 Addgene Addgene Plasmid #73021 Software and Algorithms iSTOP R package 0.1.0 This paper https://github.com/CicciaLab/iSTOP/tree/0.1.0 iSTOP reproducible analysis 0.1.1 This paper https://github.com/CicciaLab/iSTOP-paper/tree/0.1.1 R 3.4.1 The R project for statistical computing https://www.r-project.org RStudio Polymerase Chain Reaction:Article Title: CRISPR-Mediated Base Editing Enables Efficient Disruption of Eukaryotic Genes through Induction of STOP Codons Article Snippet: Article Title: CRISPR-Mediated Base Editing Enables Efficient Disruption of Eukaryotic Genes through Induction of STOP Codons Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Mouse monoclonal anti-SMARCAL1 (A-2) Santa Cruz Cat#sc-376377; RRID: AB_10987841 Mouse monoclonal anti-GAPDH Novus Cat#NB300-221; RRID: AB_10077627 Anti-Mouse IgG, HRP-linked GE Healthcare Cat#NA931-1ML; RRID: AB_772210 Bacterial and Virus Strains Subcloning Efficiency DH5α Life Technologies Cat#18265-017 Chemicals, Peptides, and Recombinant Proteins Transfection reagent: TransIT-293 Mirus Cat#MIR 2700 Carbenicillin Goldbio Cat#C-103-25 Ouabain octahydrate Sigma Cat#03125-1G Q5 High-Fidelity DNA polymerase NEB Cat#M0491L Critical Commercial Assays Quick Extract DNA Extraction solution Epicenter Cat#QE09050 Zero BLUNT II TOPO PCR Cloning kit Life Technologies Cat#450245 SuperSignal West Pico Chemiluminescent Substrate Thermo Fisher Scientific Cat#34087 Deposited Data Unprocessed images of gels and blots This paper; Mendeley Data http://dx.doi.org/10.17632/jw5rjmypy2.1 Annotated sgSTOPs for 8 organisms and 6 PAMs This paper; Mendeley Data http://www.ciccialab-database.com/istop ; http://dx.doi.org/10.17632/xbdtvf6bvj.1 Experimental Models: Cell Lines Human: HEK293T ATCC CRL-11268 Oligonucleotides Primers for PCR This paper Table S5 Oligonucleotides for sgRNA cloning This paper Table S5 Recombinant DNA Plasmid: eSpCas9(1.1) Addgene Addgene Plasmid #71814 Plasmid: MLM3636 Addgene Addgene Plasmid #43860 Plasmid: B52 (containing 2 empty sgRNAs-expressing cassettes) This paper Available in Addgene Plasmid: B270 (containing sgRNA targeting ATP1A1 + empty sgRNA-expressing cassette) This paper Available in Addgene Plasmid: pCMV-BE3 Addgene Addgene Plasmid #73021 Software and Algorithms iSTOP R package 0.1.0 This paper https://github.com/CicciaLab/iSTOP/tree/0.1.0 iSTOP reproducible analysis 0.1.1 This paper https://github.com/CicciaLab/iSTOP-paper/tree/0.1.1 R 3.4.1 The R project for statistical computing https://www.r-project.org RStudio Clone Assay:Article Title: CRISPR-Mediated Base Editing Enables Efficient Disruption of Eukaryotic Genes through Induction of STOP Codons Article Snippet: Article Title: CRISPR-Mediated Base Editing Enables Efficient Disruption of Eukaryotic Genes through Induction of STOP Codons Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Mouse monoclonal anti-SMARCAL1 (A-2) Santa Cruz Cat#sc-376377; RRID: AB_10987841 Mouse monoclonal anti-GAPDH Novus Cat#NB300-221; RRID: AB_10077627 Anti-Mouse IgG, HRP-linked GE Healthcare Cat#NA931-1ML; RRID: AB_772210 Bacterial and Virus Strains Subcloning Efficiency DH5α Life Technologies Cat#18265-017 Chemicals, Peptides, and Recombinant Proteins Transfection reagent: TransIT-293 Mirus Cat#MIR 2700 Carbenicillin Goldbio Cat#C-103-25 Ouabain octahydrate Sigma Cat#03125-1G Q5 High-Fidelity DNA polymerase NEB Cat#M0491L Critical Commercial Assays Quick Extract DNA Extraction solution Epicenter Cat#QE09050 Zero BLUNT II TOPO PCR Cloning kit Life Technologies Cat#450245 SuperSignal West Pico Chemiluminescent Substrate Thermo Fisher Scientific Cat#34087 Deposited Data Unprocessed images of gels and blots This paper; Mendeley Data http://dx.doi.org/10.17632/jw5rjmypy2.1 Annotated sgSTOPs for 8 organisms and 6 PAMs This paper; Mendeley Data http://www.ciccialab-database.com/istop ; http://dx.doi.org/10.17632/xbdtvf6bvj.1 Experimental Models: Cell Lines Human: HEK293T ATCC CRL-11268 Oligonucleotides Primers for PCR This paper Table S5 Oligonucleotides for sgRNA cloning This paper Table S5 Recombinant DNA Plasmid: eSpCas9(1.1) Addgene Addgene Plasmid #71814 Plasmid: MLM3636 Addgene Addgene Plasmid #43860 Plasmid: B52 (containing 2 empty sgRNAs-expressing cassettes) This paper Available in Addgene Plasmid: B270 (containing sgRNA targeting ATP1A1 + empty sgRNA-expressing cassette) This paper Available in Addgene Plasmid: pCMV-BE3 Addgene Addgene Plasmid #73021 Software and Algorithms iSTOP R package 0.1.0 This paper https://github.com/CicciaLab/iSTOP/tree/0.1.0 iSTOP reproducible analysis 0.1.1 This paper https://github.com/CicciaLab/iSTOP-paper/tree/0.1.1 R 3.4.1 The R project for statistical computing https://www.r-project.org RStudio Plasmid Preparation:Article Title: CRISPR-Mediated Base Editing Enables Efficient Disruption of Eukaryotic Genes through Induction of STOP Codons Article Snippet: Article Title: CRISPR-Mediated Base Editing Enables Efficient Disruption of Eukaryotic Genes through Induction of STOP Codons Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Mouse monoclonal anti-SMARCAL1 (A-2) Santa Cruz Cat#sc-376377; RRID: AB_10987841 Mouse monoclonal anti-GAPDH Novus Cat#NB300-221; RRID: AB_10077627 Anti-Mouse IgG, HRP-linked GE Healthcare Cat#NA931-1ML; RRID: AB_772210 Bacterial and Virus Strains Subcloning Efficiency DH5α Life Technologies Cat#18265-017 Chemicals, Peptides, and Recombinant Proteins Transfection reagent: TransIT-293 Mirus Cat#MIR 2700 Carbenicillin Goldbio Cat#C-103-25 Ouabain octahydrate Sigma Cat#03125-1G Q5 High-Fidelity DNA polymerase NEB Cat#M0491L Critical Commercial Assays Quick Extract DNA Extraction solution Epicenter Cat#QE09050 Zero BLUNT II TOPO PCR Cloning kit Life Technologies Cat#450245 SuperSignal West Pico Chemiluminescent Substrate Thermo Fisher Scientific Cat#34087 Deposited Data Unprocessed images of gels and blots This paper; Mendeley Data http://dx.doi.org/10.17632/jw5rjmypy2.1 Annotated sgSTOPs for 8 organisms and 6 PAMs This paper; Mendeley Data http://www.ciccialab-database.com/istop ; http://dx.doi.org/10.17632/xbdtvf6bvj.1 Experimental Models: Cell Lines Human: HEK293T ATCC CRL-11268 Oligonucleotides Primers for PCR This paper Table S5 Oligonucleotides for sgRNA cloning This paper Table S5 Recombinant DNA Plasmid: eSpCas9(1.1) Addgene Addgene Plasmid #71814 Plasmid: MLM3636 Addgene Addgene Plasmid #43860 Plasmid: B52 (containing 2 empty sgRNAs-expressing cassettes) This paper Available in Addgene Plasmid: B270 (containing sgRNA targeting ATP1A1 + empty sgRNA-expressing cassette) This paper Available in Addgene Plasmid: pCMV-BE3 Addgene Addgene Plasmid #73021 Software and Algorithms iSTOP R package 0.1.0 This paper https://github.com/CicciaLab/iSTOP/tree/0.1.0 iSTOP reproducible analysis 0.1.1 This paper https://github.com/CicciaLab/iSTOP-paper/tree/0.1.1 R 3.4.1 The R project for statistical computing https://www.r-project.org RStudio Software:Article Title: CRISPR-Mediated Base Editing Enables Efficient Disruption of Eukaryotic Genes through Induction of STOP Codons Article Snippet: Article Title: CRISPR-Mediated Base Editing Enables Efficient Disruption of Eukaryotic Genes through Induction of STOP Codons Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Mouse monoclonal anti-SMARCAL1 (A-2) Santa Cruz Cat#sc-376377; RRID: AB_10987841 Mouse monoclonal anti-GAPDH Novus Cat#NB300-221; RRID: AB_10077627 Anti-Mouse IgG, HRP-linked GE Healthcare Cat#NA931-1ML; RRID: AB_772210 Bacterial and Virus Strains Subcloning Efficiency DH5α Life Technologies Cat#18265-017 Chemicals, Peptides, and Recombinant Proteins Transfection reagent: TransIT-293 Mirus Cat#MIR 2700 Carbenicillin Goldbio Cat#C-103-25 Ouabain octahydrate Sigma Cat#03125-1G Q5 High-Fidelity DNA polymerase NEB Cat#M0491L Critical Commercial Assays Quick Extract DNA Extraction solution Epicenter Cat#QE09050 Zero BLUNT II TOPO PCR Cloning kit Life Technologies Cat#450245 SuperSignal West Pico Chemiluminescent Substrate Thermo Fisher Scientific Cat#34087 Deposited Data Unprocessed images of gels and blots This paper; Mendeley Data http://dx.doi.org/10.17632/jw5rjmypy2.1 Annotated sgSTOPs for 8 organisms and 6 PAMs This paper; Mendeley Data http://www.ciccialab-database.com/istop ; http://dx.doi.org/10.17632/xbdtvf6bvj.1 Experimental Models: Cell Lines Human: HEK293T ATCC CRL-11268 Oligonucleotides Primers for PCR This paper Table S5 Oligonucleotides for sgRNA cloning This paper Table S5 Recombinant DNA Plasmid: eSpCas9(1.1) Addgene Addgene Plasmid #71814 Plasmid: MLM3636 Addgene Addgene Plasmid #43860 Plasmid: B52 (containing 2 empty sgRNAs-expressing cassettes) This paper Available in Addgene Plasmid: B270 (containing sgRNA targeting ATP1A1 + empty sgRNA-expressing cassette) This paper Available in Addgene Plasmid: pCMV-BE3 Addgene Addgene Plasmid #73021 Software and Algorithms iSTOP R package 0.1.0 This paper https://github.com/CicciaLab/iSTOP/tree/0.1.0 iSTOP reproducible analysis 0.1.1 This paper https://github.com/CicciaLab/iSTOP-paper/tree/0.1.1 R 3.4.1 The R project for statistical computing https://www.r-project.org RStudio PCR Cloning:Article Title: CRISPR-Mediated Base Editing Enables Efficient Disruption of Eukaryotic Genes through Induction of STOP Codons Article Snippet: Article Title: CRISPR-Mediated Base Editing Enables Efficient Disruption of Eukaryotic Genes through Induction of STOP Codons Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Mouse monoclonal anti-SMARCAL1 (A-2) Santa Cruz Cat#sc-376377; RRID: AB_10987841 Mouse monoclonal anti-GAPDH Novus Cat#NB300-221; RRID: AB_10077627 Anti-Mouse IgG, HRP-linked GE Healthcare Cat#NA931-1ML; RRID: AB_772210 Bacterial and Virus Strains Subcloning Efficiency DH5α Life Technologies Cat#18265-017 Chemicals, Peptides, and Recombinant Proteins Transfection reagent: TransIT-293 Mirus Cat#MIR 2700 Carbenicillin Goldbio Cat#C-103-25 Ouabain octahydrate Sigma Cat#03125-1G Q5 High-Fidelity DNA polymerase NEB Cat#M0491L Critical Commercial Assays Quick Extract DNA Extraction solution Epicenter Cat#QE09050 Zero BLUNT II TOPO PCR Cloning kit Life Technologies Cat#450245 SuperSignal West Pico Chemiluminescent Substrate Thermo Fisher Scientific Cat#34087 Deposited Data Unprocessed images of gels and blots This paper; Mendeley Data http://dx.doi.org/10.17632/jw5rjmypy2.1 Annotated sgSTOPs for 8 organisms and 6 PAMs This paper; Mendeley Data http://www.ciccialab-database.com/istop ; http://dx.doi.org/10.17632/xbdtvf6bvj.1 Experimental Models: Cell Lines Human: HEK293T ATCC CRL-11268 Oligonucleotides Primers for PCR This paper Table S5 Oligonucleotides for sgRNA cloning This paper Table S5 Recombinant DNA Plasmid: eSpCas9(1.1) Addgene Addgene Plasmid #71814 Plasmid: MLM3636 Addgene Addgene Plasmid #43860 Plasmid: B52 (containing 2 empty sgRNAs-expressing cassettes) This paper Available in Addgene Plasmid: B270 (containing sgRNA targeting ATP1A1 + empty sgRNA-expressing cassette) This paper Available in Addgene Plasmid: pCMV-BE3 Addgene Addgene Plasmid #73021 Software and Algorithms iSTOP R package 0.1.0 This paper https://github.com/CicciaLab/iSTOP/tree/0.1.0 iSTOP reproducible analysis 0.1.1 This paper https://github.com/CicciaLab/iSTOP-paper/tree/0.1.1 R 3.4.1 The R project for statistical computing https://www.r-project.org RStudio Virus:Article Title: CRISPR-Mediated Base Editing Enables Efficient Disruption of Eukaryotic Genes through Induction of STOP Codons Article Snippet: Article Title: CRISPR-Mediated Base Editing Enables Efficient Disruption of Eukaryotic Genes through Induction of STOP Codons Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Mouse monoclonal anti-SMARCAL1 (A-2) Santa Cruz Cat#sc-376377; RRID: AB_10987841 Mouse monoclonal anti-GAPDH Novus Cat#NB300-221; RRID: AB_10077627 Anti-Mouse IgG, HRP-linked GE Healthcare Cat#NA931-1ML; RRID: AB_772210 Bacterial and Virus Strains Subcloning Efficiency DH5α Life Technologies Cat#18265-017 Chemicals, Peptides, and Recombinant Proteins Transfection reagent: TransIT-293 Mirus Cat#MIR 2700 Carbenicillin Goldbio Cat#C-103-25 Ouabain octahydrate Sigma Cat#03125-1G Q5 High-Fidelity DNA polymerase NEB Cat#M0491L Critical Commercial Assays Quick Extract DNA Extraction solution Epicenter Cat#QE09050 Zero BLUNT II TOPO PCR Cloning kit Life Technologies Cat#450245 SuperSignal West Pico Chemiluminescent Substrate Thermo Fisher Scientific Cat#34087 Deposited Data Unprocessed images of gels and blots This paper; Mendeley Data http://dx.doi.org/10.17632/jw5rjmypy2.1 Annotated sgSTOPs for 8 organisms and 6 PAMs This paper; Mendeley Data http://www.ciccialab-database.com/istop ; http://dx.doi.org/10.17632/xbdtvf6bvj.1 Experimental Models: Cell Lines Human: HEK293T ATCC CRL-11268 Oligonucleotides Primers for PCR This paper Table S5 Oligonucleotides for sgRNA cloning This paper Table S5 Recombinant DNA Plasmid: eSpCas9(1.1) Addgene Addgene Plasmid #71814 Plasmid: MLM3636 Addgene Addgene Plasmid #43860 Plasmid: B52 (containing 2 empty sgRNAs-expressing cassettes) This paper Available in Addgene Plasmid: B270 (containing sgRNA targeting ATP1A1 + empty sgRNA-expressing cassette) This paper Available in Addgene Plasmid: pCMV-BE3 Addgene Addgene Plasmid #73021 Software and Algorithms iSTOP R package 0.1.0 This paper https://github.com/CicciaLab/iSTOP/tree/0.1.0 iSTOP reproducible analysis 0.1.1 This paper https://github.com/CicciaLab/iSTOP-paper/tree/0.1.1 R 3.4.1 The R project for statistical computing https://www.r-project.org RStudio Cloning:Article Title: CRISPR-Mediated Base Editing Enables Efficient Disruption of Eukaryotic Genes through Induction of STOP Codons Article Snippet: Article Title: CRISPR-Mediated Base Editing Enables Efficient Disruption of Eukaryotic Genes through Induction of STOP Codons Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Mouse monoclonal anti-SMARCAL1 (A-2) Santa Cruz Cat#sc-376377; RRID: AB_10987841 Mouse monoclonal anti-GAPDH Novus Cat#NB300-221; RRID: AB_10077627 Anti-Mouse IgG, HRP-linked GE Healthcare Cat#NA931-1ML; RRID: AB_772210 Bacterial and Virus Strains Subcloning Efficiency DH5α Life Technologies Cat#18265-017 Chemicals, Peptides, and Recombinant Proteins Transfection reagent: TransIT-293 Mirus Cat#MIR 2700 Carbenicillin Goldbio Cat#C-103-25 Ouabain octahydrate Sigma Cat#03125-1G Q5 High-Fidelity DNA polymerase NEB Cat#M0491L Critical Commercial Assays Quick Extract DNA Extraction solution Epicenter Cat#QE09050 Zero BLUNT II TOPO PCR Cloning kit Life Technologies Cat#450245 SuperSignal West Pico Chemiluminescent Substrate Thermo Fisher Scientific Cat#34087 Deposited Data Unprocessed images of gels and blots This paper; Mendeley Data http://dx.doi.org/10.17632/jw5rjmypy2.1 Annotated sgSTOPs for 8 organisms and 6 PAMs This paper; Mendeley Data http://www.ciccialab-database.com/istop ; http://dx.doi.org/10.17632/xbdtvf6bvj.1 Experimental Models: Cell Lines Human: HEK293T ATCC CRL-11268 Oligonucleotides Primers for PCR This paper Table S5 Oligonucleotides for sgRNA cloning This paper Table S5 Recombinant DNA Plasmid: eSpCas9(1.1) Addgene Addgene Plasmid #71814 Plasmid: MLM3636 Addgene Addgene Plasmid #43860 Plasmid: B52 (containing 2 empty sgRNAs-expressing cassettes) This paper Available in Addgene Plasmid: B270 (containing sgRNA targeting ATP1A1 + empty sgRNA-expressing cassette) This paper Available in Addgene Plasmid: pCMV-BE3 Addgene Addgene Plasmid #73021 Software and Algorithms iSTOP R package 0.1.0 This paper https://github.com/CicciaLab/iSTOP/tree/0.1.0 iSTOP reproducible analysis 0.1.1 This paper https://github.com/CicciaLab/iSTOP-paper/tree/0.1.1 R 3.4.1 The R project for statistical computing https://www.r-project.org RStudio |